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in scientific fiction and reality  virus is a June premiere  virus was the most mysterious subject in medicine  bacterial filter discovery electron microscope we unveil this mystery

in this course we speak about history of discovery virus structure virus classifications  virus multiplication  virus pathogenesis zoonosis some important viruses hepatitis

Rabies is a vaccine preventable, zoonotic, viral disease. Once clinical symptoms appear, rabies is. Vaccinating dogs is the most

cost effective strategy for preventing rabies in people. post

exposure prophylaxis Post exposure prophylaxis(PEP) is the immediate treatment of a bite victim after rabies exposure.  This prevents virus entry into

the central nervous system, which results in imminent death. PEP consists of:•Extensive washing and local treatment of the bite wound or

scratch as soon as possible after a suspected exposure;•a course of potent and effective rabies vaccine and the administration of rabies immunoglobulin (RIG), if indicated

Laboratory diagnosis of viral infections  v1-Sampling

2-Virus isolation

3-Nucleic acid based methods

polymerase chain reaction

sequencingv4-Microscopy based methods immunofluorescence Electron microscopy 5-Host antibody detection 6-Hemagglutination assay

sampling temperatures (usually 4 °C) to preserve the virus and prevent bacterial or fungal growth. Sometimes multiple sites may also be sampled.

Types of samples include the following    A wide variety of samples can be used for virologic testing. The type of sample sent to the laboratory often depends on the type of viral infection being diagnosed and the test required  Proper sampling technique is essential to avoid potential pre-analytical errors .For example and stored at appropriate    stored at appropriate temperatures (usually 4 °C) to preserve the virus and prevent bacterial or fungal growth.  1.Nasopharyngeal swab

2.Blood  skin. Sputum, gargles and bronchial washings .Urine .Semen Faces  .Cerebrospinal fluid   .Tissues biopsies or post-mortem

Viruses are often isolated from the initial patient sample.   This allows the virus sample to be grown into larger quantities and allows a larger number of tests to be run on them. This is particularly important for samples that contain new or rare viruses for which diagnostic tests are not yet developed  Many viruses can be grown in cell culture in the lab.

To do this, the virus sample is mixed with cells, a process called adsorption, after which the cells become infected and produce more copies of the virus   Although different viruses often only grow in certain types of cells, there are cells that support the growth of a large variety of viruses and are a good starting point, for example, the African monkey kidney cell line (Vero cells), human lung fibroblasts (MRC-5), and human epidermoid carcinoma cells (HEp-2). One means of determining whether the cells are successfully  replicating the virus is to check for a change in cell morphology or for the presence of cell death using a microscope  Other viruses may require alternative methods for growth such as the inoculation of embryonated chicken eggs